Saxs-labdisk: a Centrifugal Microfluidic Screening Platform for Protein Structure Analysis
نویسندگان
چکیده
A small angle x-ray scattering (SAXS) screening platform for fully automated protein structure analysis based on a centrifugal microfluidic LabDisk is presented. Protein sample (2 μl), dilution buffer (3 μl), and screening solution (3 μl) are split into 40 nl aliquots each (CVs < 5.5%), recombined in predefined ratios to 20 samples of 240 nl and mixed. On-Disk analysis can then be performed in a SAXS beamline. Up to 7 different screenings can be performed in parallel on one disk. For the first time, the SAXS-LabDisk will enable routine SAXS screening of minute protein volumes.
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تاریخ انتشار 2012